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. 2003 Jan 27;100(4):1598–1602. doi: 10.1073/pnas.0337661100

Figure 1.

Figure 1

Expression of EGSRpp38 in HeLa cells leads to the reduction of Rpp38 mRNA and Rpp38 protein. HeLa cells were transiently transfected with 10 μg of empty (no EGS) pmU6 or pmU6- EGSRpp38 plasmid. Samples of total RNA and total protein were prepared from these HeLa cells 0, 12, 24, 48, and 60 h after transfection. For Northern blot analysis of Rpp38 mRNA and EGSRpp38, RNA samples (10 μg) of total RNA were hybridized with end-labeled antisense oligonucleotide that corresponded to Rpp38 mRNA (A) and EGSRpp38 RNA (B). Lane H, sample of total RNA, prepared from nontransfected HeLa cells. Lane C, 0.1 pmol of Rpp38 mRNA fragment (A) and 0.1 pmol of EGSRpp38 RNA (B), transcribed in vitro. For Western blot analysis, samples (10 μg) of total protein were immunoblotted with a rabbit polyclonal antibodies against Rpp38 (1:100 dilution) (C and D). The lanes in C and D represent cells harvested after the given number of hours after transfection.