Figure 2.

The inhibition of Rpp38 expression caused by EGS^Rpp38 in HeLa cells influences the expression of some other RNase P protein subunits in vivo. Samples of total protein (10 μg each lane) were prepared from the HeLa cells, transiently transfected by 10 μg of pmU6-EGS^Rpp38-TL plasmid for 24 h (lane 1) and 10 μg of pmU6-EGS^Rpp38 plasmid for 24 h (lane 2) and 72 h (lane 3). For Western blot analysis, these samples were separated in a 12.5% polyacrylamide/SDS gel, transferred to nitrocellulose filters, and immunoblotted with polyclonal antibodies against the indicated RNase P protein subunits (1:100 dilution), β-actin (1:500), and lamin A (1:500).