Figure 3.

Expression of EGS^Rpp38 decreases the amount of Rpp25 mRNA and does not change the amount of Rpp40 mRNA in vivo. HeLa cells were transiently transfected by pmU6 or pmU6-EGS^Rpp38 plasmids and harvested 0, 12, 24, 48, and 60 h after transfection. Samples (10 μg) of total RNA isolated from these cells were hybridized with the end-labeled antisense oligonucleotide that corresponded to Rpp25 RNA (A), Rpp40 RNA (B), H1 RNA (C), and MRP RNA (D). Lane H, total RNA, isolated from nontransfected HeLa cells. Lane C, 0.05 pmol of H1 RNA (C) and 0.2 pmol of MRP RNA (D), transcribed in vitro.