Figure 4.

Rpp38, Rpp25, and Rpp21 are absent from the cell nucleolus after expression of EGS^Rpp38 in vivo. HeLa cells grown in six-well plates transfected with 2 μg of empty pmU6 plasmid (A, C, and E) or pmU6-EGS^Rpp38 plasmid (B, D, and F) were grown for 24 h and then examined for the presence of Rpp38 (A and B), Rpp25 (C and D), and Rpp21 (E and F) in indirect immunofluorescence analysis. All images were acquired during the same experimental observation. No specific signal was observed when control preimmune sera of rabbits were tested (data not shown). (Scale bar: 20 μm).