Table 1.
Relative amounts of mRNA and proteins of RNase P subunits after transfection with pmU6 or pmU6EGSRpp38
| Gene product | pmU6/pmU6-EGSRpp38
|
||
|---|---|---|---|
| cDNA | Western blot | Northern blot | |
| Rpp14 | 1.2 | ||
| Rpp20 | 0.2 | 1 | |
| Rpp21 | 0.14 | 0.4 | |
| Rpp25 | 0.09 | 0.4 | 0.4 |
| Rpp29 | 0.17 | 0.6 | |
| Rpp30 | 1 | 1 | |
| Rpp38 | 0.12 | 0.4 | 0.2 |
| Rpp40 | 0.5 | 1 | 1 |
| hpop5 | 0.18 | ||
| hpop1 | 0.25 | ||
| Lamin A | 0.5 | ||
| β-actin | 1.1 | 1 | |
| H1 RNA | 1.1 | 1 | |
| MRP RNA | 1 | ||
| 5S RNA | 1 | ||
| pre-tRNALeu/tRNALeu | 0.8 | ||
cDNA results were quantitated by using data obtained from the controls in Fig. 5 (see Materials and Methods). A result of at least <0.2 was regarded as reliably lower than the control of no change. In each column, the results were normalized to controls. Western blots (Fig. 2) were also quantitated but the results are not as reliable as shown for some Northerns (Figs. 1 and 3). The data shown, however, are quite clear for the bands that show a decrease with respect to unchanged bands. A Northern blot was quantitated for precursor and tRNA (data not shown) to make a rough estimate of their amounts.