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van't Hoff analysis of Compound 1 binding to IL-2. Kd values were determined by SPR at four temperatures (see Methods). (ΔH = −8.9 ± 0.35 kcal/mol, ΔS =−6.8 ± 0.56 cal/mol K.) Data are an average of three measurements; the standard deviations for each temperature are shown on the graph.
