Figure 6.

Differential localization of GFP lamin B3 tail chimeras. HeLa cells transiently transfected with GFP lamin tail chimeras were fixed 24 h after transfection and examined by fluorescence confocal laser scan microscopy. Schemes of the chimeras are given at the top. The nomenclature of the chimeras corresponds to that of the lamin constructs listed in Table 1. The basic cluster and the palmitoylation site both contribute to NE targeting (C–E, I, and K). Targeting to the NE is most efficient in the presence of both motifs (C and I). The basic cluster acts as a NLS (I, K, and L). Constructs that lack a NLS but contain a palmitoylation site and a CaaX motif are associated with the PM, the Golgi, and peri-Golgi vesicles (H and J). Arrows in C indicate NE indentations decorated by the GFP-B3bwt chimera. Bar in L, 50 μm.