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Subcellular localization of FRNK mutants. Control CE cells (A), or CE cells expressing FRNK (B), FRNKE291/K295 (C), FRNKE289/N298D/D300 (D), FRNKV261/L268 (E), or FRNKH332/K339 (F) were plated on glass coverslips. The cells were fixed, permeabilized, and stained with mAb 2A7. The mAb was detected with rhodamine-labeled anti-mouse IgG antibody.
