Figure 9.

Induction of tyrosine phosphorylation of paxillin by FAK mutants. (A and C) Control CE cells (lanes 1 and 2) or CE cells expressing wild-type, epitope-tagged FAK (lanes 3 and 4) or FAK^{E949/K956/R963} (A, lanes 5 and 6), FAK^V955/L962 (A, lanes 7 and 8), FAK^E985/K989 (A, lanes 9 and 10), FAK^L995/L998 (C, lanes 5 and 6), FAK^H1026/K1033 (C, lanes 7 and 8), or FAK^L1028/L1035 (C, lanes 9 and 10) were left untreated (odd lanes) or treated with pervanadate (even lanes). The cells were lysed and paxillin was immunoprecipitated and Western blotted for phosphotyrosine (A and C, top). The blot was striped and reprobed for paxillin (A and C, bottom). (B and D), Twenty-five micrograms of cell lysate was analyzed for FAK expression by Western blotting with BC4.