Dependence of the ratio of reverse and forward export on the passive diffusion rate of export cargoes. DHFR-An3, Rev, An3, and NS2 stand for b-DHFR-An3-GST, b-Rev-GST, b-NS2-GST, and b-An3-GST constructs, respectively. The values are from Table 1. The position of b-DHFR-An3-GST is approximate because the rate of reverse export of this construct is not accurately determined (<0.005 min−1).