Figure 2.

Telomere length in wild-type and pku70⁻ strains. Genomic DNA of pku70⁺ trt1⁺ and pku70⁻ trt1⁺ strains was prepared on the indicated days of monitored growth in liquid culture (lanes a–e) or after three and seven restreaks on plates (lanes f–h). A total of 15 μg of DNA from each sample was digested with EcoRI and subjected to agarose gel electrophoresis and Southern transfer onto a nylon membrane. The blot was subsequently probed with a ³²P-labeled telomeric fragment. As a loading control, a ³²P-labeled fragment of the single-copy pol1⁺ gene was included in the hybridization mix. Size markers were 100-bp and 1-kb ladders from New England Biolabs.