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. 2000 Oct;11(10):3265–3275. doi: 10.1091/mbc.11.10.3265

Figure 6.

Figure 6

Chromosome circularization in trt1− and pku70− trt1− strains. (A) Scheme of NotI restriction sites in S. pombe chromosomes. The terminal fragments on chromosomes I and II are shown in gray. Chromosome III lacks a NotI restriction site. (B and C) Pulsed-field gel analysis of NotI-digested genomic DNA from the same trt1− and pku70− trt1− strains used in Figure 5. The gel was run and processed as described in MATERIALS AND METHODS. DNA was transferred onto a nylon membrane and hybridized to internal probes on the I, L, M, and C fragments. The terminal fragments of linear chromosomes I and II are indicated on the left, and the fragments resulting from chromosome circularization (I+L and C+M) are indicated on the right.