Figure 1.

(A) Tissue distribution of mouse ICA69. A total of 40 μg of protein from the various tissues was loaded in each lane. The predominant ICA69 band is indicated by the arrow. (B) Subcellular fractionation of mouse brain and Western blot detection of ICA69, VAMP-2, NMDAR, SUMO-1, and β-tubulin is shown. The nature of the different fractions is as follows: S1, postnuclear supernatant; P2, crude synaptosomes; P3, microsomes; S3, cytosolic soluble; LP1, plasma membranes of synaptosomes; LP2, synaptic vesicles. A total of 40 μg of protein was loaded in each lane.