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(A) Structure of the ric-19 gene as well as the approximate positions of the primers used to screen and isolate a deletion mutation spanning positions −354 to 2276. (B) Example of single-worm PCR reactions with the use of the primers AR1 and AL1 on N2 worms (lanes 1–4) or worms homozygous for the ric-19(pk690) allele (lanes 5–8). The arrowhead and arrow in B indicate the amplified wild-type and pk690 alleles of ric-19, respectively.
