Skip to main content
. 2000 Oct;11(10):3289–3298. doi: 10.1091/mbc.11.10.3289

Figure 2.

Figure 2

Immunogold localization of endobrevin and LIMP II. Ultrathin cryosections of NRK cells were immunolabeled for endobrevin (15-nm protein A–gold) and either LIMP II (10-nm protein A–gold; A) or the endogenous MPR46 (10-nm protein A–gold; B and C). Endobrevin is detectable in tubulovesicular structures (arrows in A), underneath the plasma membrane (PM), and on endosomal vacuoles (E) and endosomes that appear as multivesicular bodies (MVB), where it often colocalizes with the lysosomal membrane protein LIMP II. We also noted a colocalization of endobrevin with MPR46 in TGN-associated structures (B, arrowheads) and occasionally the appearance in coated pits/vesicles at the plasma membrane (C, arrow). Bars, 100 nm.