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. 2000 Oct;11(10):3289–3298. doi: 10.1091/mbc.11.10.3289

Figure 6.

Figure 6

Fusion of early and late endosomes is inhibited by Fab fragments specific for endobrevin or by recombinant endobrevin. Fusion of early and late endosomes derived from PC12 cells was monitored in vitro with the use of a content-mixing assay (see MATERIALS AND METHODS). For preincubation of PNS, 1.2 μM Fab fragments specific for endobrevin (eb) or synaptophysin (syp) (left panels) or 30 μM soluble recombinant endobrevin or synaptobrevin (sb) (right panels) was used. ATP-dependent fusion activity in the absence of Fab fragments or recombinant protein is defined as 100%. Values are given as means of four independent experiments, and bars indicate the range. Fusion was initiated by mixing donor and acceptor fractions while simultaneously adding ATP and cytosol, followed by 30 min of incubation.

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