Table 1.

Quantification of endobrevin in NRK cells

Compartment	Percent gold particles	Of these, percent positive for LIMP II	LIMP II in NRK
Golgi/TGN	12	0	5
Tubules, vesicles not assigned to TGN or PM	18	14	8
Tubulovesicular structures near PM	26	15	8
PM	8	0	2
MVBs/vacuolar endosomes, ATVs	29	71	72
Unclassified	7	0	5

The cells were fixed and labeled for endobrevin and LIMP II as described in MATERIALS AND METHODS. For quantification, 34 sections were analyzed, and a total of 2800 gold particles were counted. PM, plasma membrane; MVBs, multivesicular bodies; ATVs, associated tubules and vesicles.