Table 1.
Sequences of primers and probes used in the PCR and quantitative real-time PCR analyses
| Region | Forward primer (5′–3′) | Reverse primer (5′–3′) | TaqMan probe (5′–3′) |
|---|---|---|---|
| D-loop | tatcttttggcggtatgcacttttaacagt | tgatgagattagtagtatgggagtgg | caccccccaactaacacattattttcccc |
| (401–430) | (461–490) | (431–459) | |
| D310 | cacacagacatcataacaaaaaatttcc | ggtgttagggttctttgtttttgg | ccccccctcccccgcttct |
| (269–296) | (355–378) | (303–321) | |
| COII | ccccacattaggcttaaaaacagat | accgctacacgaccgggggtata | caattcccggacgtctaaaccaaaccactttc |
| (8,080–8,103) | (8,138–8,160) | (8,105–8,136) | |
| tRNAG | gccacctatcacaccccatc | tacccttcccgtactaattaatccc | gctatcgggcccataccccgaaaatgt |
| (4,378–4,397) | (4,454–4,478) | (4,420–4,446) | |
| β-Actin | tcacccacactgtgcccatctacga | cagcggaaccgctcattgccaatgg | atgccccccccatgccatcctgcgt |
| (2,141–2,165) | (2,411–2,435) | (2,171–2,196) | |
| Mt35A | ggagctctccatgcatttgg | gggtgatgtgagcccgtcta | |
| (35–53) Mt35F | (620–639) Mt635R | ||
| F57A | acaattgaatgtctgcacagccactt | ataccccaatcgtcgccacaca | |
| (241–266) F57A | (519–540) R15i2 |