Figure 1.

Allergy-related gene expression in CNS of mice with EAE and in Th1 and Th2 TCL activated against a myelin peptide. (a–f) EAE was induced with PLP 139–151 in SJL mice; brain and spinal cord were removed at different time points of the disease and analyzed by quantitative PCR. The relative expression of PAFR (a and b), PGDS (c and d), and MMCP-7 (e and f) was quantified by using primers specific for the target (see Materials and Methods) and normalization against β-actin. Means of qPCR values of three to five animals ± SD per time point are represented. *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.001 by ANOVA vs. naive. (g) The relative expression of H1R and H2R was quantified in a Th1 and Th2 type TCL specific for PLP 139–151. Data are representative of two consecutive experiments. *, P < 0.05, **, P < 0.01 by ANOVA Th1 vs. Th2.