Figure 2.

Expression of H1R and H2R in the CNS of SJL mice with EAE induced with PLP 139–151. Brains were obtained 20 days after disease induction, and cryostat sections were stained with rabbit polyclonal antibodies against H1R and H2R. H1R (A) and H2R (B) are expressed on mononuclear cells (arrowheads) in perivascular inflammatory foci. Parenchymal cells consistent with microglia, astrocytes, and infiltrating inflammatory cells (arrows) are also stained. In brains of naive SJL mice, H1R (C) is not detected, although rare astrocytes and choroid plexus cells were stained (not shown). H2R (D) is expressed on microvascular endothelial cells (arrows). Original magnifications: A and C, ×240; B and D, ×320.