Table 3.

Serum antibody responses and allergic reactions to MOG 35–55 in FcγRIII^−/−, in FcRγ chain −/− and their controls

Strain	Antibody responses*			No. of mice with allergic reactions at challenge†
	IgG1, OD405	IgG2a, OD405	Total IgE, μg/ml
FcγRIII−/−	1.094  ± 0.473	0.123  ± 0.053	4.2  ± 0.23	6/12
FcγRIII+/+	0.259  ± 0.047	0.048  ± 0.021	1.45  ± 0.47	7/8
FcR γ chain −/−	1.343  ± 0.506	0.140  ± 0.074	4.1  ± 0.17	0/11
FcR γ chain +/+	0.434  ± 0.179	0.071  ± 0.022	3.45  ± 0.51	5/9

^*Serum from individual mice (5–12 per group) was collected 6 wk after the induction of EAE and tested individually by ELISA. Numbers represent mean ± SEM. 

^† Challenge was 6 wk after the induction of EAE with MOG 35–55 (0.1 mg) in PBS i.p., and presence of allergic reactions was confirmed by a reduction in body temperature of at least 0.5°C (see Materials and Methods).