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. 2003 Feb 5;100(4):1879–1884. doi: 10.1073/pnas.0237137100

Figure 5.

Figure 5

STAT3 regulates crucial functional responses of the innate immune system. (a) IκB protein and phosphorylation levels were measured by Western blotting with specific antibodies to IκB and phospho-IκB before and after 10-min and 1-h LPS (10 ng/ml) stimulation of bone marrow-derived myeloid cells cultured in granulocyte/macrophage-CSF as indicated (FF, control mice; CFF, STAT3-CFF mice). (b) NADPH oxidase activity was measured in neutrophils isolated from whole blood samples of 4- to 5-week-old mice. Five controls (shaded bar) were compared with five sex-matched littermates (hatched bars). The difference between control and STAT3 knockout mice was statistically significant (five pairs analyzed). P value of Student's t test was P < 0.004. (c) Sera from 4- to 5-week-old mice were collected. IFN-γ, IL-4, tumor necrosis factor α (TNF-α), and IL-5 levels were measured. The results with three matched pairs of control (shaded bars) and STAT3-CFF littermates (open bars) are shown.