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Nuclear accumulation of Ajuba LIM domains is required to induce endodermal differentiation of P19 cells. Indirect immunofluorescence of transiently transfected fibroblasts (A–D) or stable P19 clones (E–L) with anti-myc antiserum (A and C), anti-human IL2 receptor α antiserum (B and D), mAb SSEA 1 (E–H), or mAb TROMA-1 (I–L). Cells contain LIM 1,2,3 (A, E, and I), hIL2Rα–LIM 1,2,3 (B, F, and J), Δ289–297 (C, G, and K), or hIL2Rα–Δ289–297 (D, H, and L).
