Table 1.
Activation of JNK was required for Ajuba-mediated induction of endodermal differentiation of P19 cells
| JNK activity | Endodermal differentiation
|
||
|---|---|---|---|
| P19.Neo | P19.JNK1–APF | ||
| Retinoic acid (10 nM) | + | + | − |
| Ajuba | − | − | − |
| PreLIM | + | − | nd |
| LIM 1,2,3 | + | + | − |
| Δ289–297 | + | + | − |
| LIM 3 | + | + | − |
JNK activity was determined by in vitro kinase assay as described. The state of differentiation of P19 cells was determined by immunofluorescence analysis: ectodermal, SSEA 1 positive, TROMA-1 negative; endodermal, SSEA 1 negative, TROMA-1 positive. nd, not determined.