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Relative expression of the EAAT2-Prom in PHFA and various normal and tumor cell lines. (A) Relative fold expression of a FL-EAAT2-Prom-LUC construct was determined as described (28, 30), and activity versus transfection with a pSV-β-galactosidase plasmid, to equalize for differences in transfection efficiency, was determined (28, 30). (B) Effect of passage number on EAAT2-Prom-LUC activity in PHFA cells.
