Skip to main content
. 2003 Feb 10;100(4):1955–1960. doi: 10.1073/pnas.0136555100

Figure 4.

Figure 4

Effect of modulators of EAAT2 activity on EAAT2 expression and EAAT2-Prom deletion mutant activity in PHFA. (A) Northern analysis of EAAT2 and GAPDH mRNA expression after treatment with EGF (30 ng/ml), TGF-α (30 ng/ml), dBcAMP (200 μM), bromo-cAMP (250 μM), or TNF-α (200 units/ml) for 7 days. (B) Nuclear run-on assays determining relative rate of EAAT2 and GAPDH transcription as a function of 7-day treatment with the same concentration of the indicated agents. (C) Time-course mRNA expression of EAAT2 and GAPDH after treatment with EGF, bromo-cAMP, or TNF-α (same concentrations as in A). (D) Effect of deletion mutations in the EAAT2-Prom on basal and modulator-regulated expression. Relative fold expression of the p-703, p-326, and p-120 in untreated and 4-day compound-treated PHFA. (E) Effect of defined mutations in the EAAT2-Prom on basal, EGF, and TNF-α treatment. PHFA were treated for 4 days, and results represent relative fold expression.