Differential induction of IL-8 secretion in transfected HEK cells by ethanol-killed vs. heat-killed S. pneumoniae. HEK 293 cells stably transfected with CD14, TLR2, or TLR4 were stimulated over 18 h with medium (white bars), 10 ng/ml LPS (gray bars), ethanol-killed pneumococcus (black bars), and heat-killed pneumococcus (hatched bars).