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Nuclear translocation of NF-κB due to pneumolysin is TLR4-dependent. Peritoneal macrophages from C57BL/6 or TLR4−/− mice were stimulated for 1 h with medium, 10 ng/ml LPS, 5 μg/ml lipoprotein (Pam3Cys-Ser-Lys-4), or 10 μg/ml pneumolysin, and then nuclear extracts were prepared and analyzed by EMSA.
