Figure 4.

Genes encoding GAS prophage and secreted proteins. (A) Prophage-encoded genes differentially regulated during phagocytosis. Genes were assigned to prophage (red) based on homology with serotype M1 GAS SF370 (11). (B) Differential expression of genes encoding secreted proteins of known (left) and unknown (right) function. Length of arrows is relative for gene expression. (C) Production of novel secreted proteins in humans with GAS disease. SPy0136 and SPy2191 were overexpressed in E. coli and separated by SDS-PAGE (Gel-Code protein stain, Left). Comparison of acute (Ac) and convalescent (Cv) paired sera from an individual with GAS invasive disease (Right). Data are representative of paired human sera from three individuals with GAS invasive disease.