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. 2000 Oct;11(10):3329–3340. doi: 10.1091/mbc.11.10.3329

Figure 3.

Figure 3

TEP1 binding of hTERT truncations. (A) hTERT truncations and full-length TEP1 (containing a myc-epitope tag) were cotransfected into human 293T cells. Cell extracts were immunoprecipitated by using an anti-flag antibody (to immunoprecipitate hTERT, bottom) and then analyzed by Western analysis by using anti-myc antibody to examine coimmunoprecipitation of TEP 1 (top). (B) Full-length hTERT was synthesized in RRL in the presence of 0.002 μg/μl purified hTER. The lysate alone (no DNA), containing full-length hTERT (TERT) or containing the RT domain (601–927) was incubated on ice either alone (alone, lanes 1 and 4), with mock 293T-transfected cell extracts (+mock, lanes 2 and 5), or TEP1 (with a myc epitope tag) transfected 293T cell extracts (+TEP1, lanes 3 and 6). The mixtures were then immunoprecipitated with anti-flag antibody and assayed for telomerase activity (top) and blotted for the presence of hTERT by using an anti-hTERT antibody (Western blot, middle) and TEP1 by using an anti-myc antibody (Western blot, bottom).