Figure 5.

Primer use by full-length and 201-1132 hTERT. Anti-flag immunoprecipitations containing full-length hTERT (FL), an hTERT truncation spanning amino acids 201-1132 (201), or lysate only (mock), from either RRL (lanes 1–5 and 11–15) or transfected human 293T cells (293T; lanes 6–10 and 16–20) were analyzed for telomerase activity with 0.1 μg of either the TS primer (lanes 1–10) or a TS primer that lacked the 3′ GTT nucleotides (−3′ GTT, lanes 11–20). The triangles represent 2-fold dilutions of each of the immunoprecipitates to demonstrate that the telomerase reactions were in the linear range.