Figure 6.

RNA binding and catalytic activities of hTERT and hTER truncation combinations. (A) Northern analysis (as in Figure 2C) of each in vitro transcribed hTER truncation (5 ng) before the addition of RRL. Numbers of the hTER truncations refer to nucleotide positions. (B) Full-length hTERT or 301-1132 hTERT was synthesized in RRL in the presence of different hTER truncations (0.01 μg/μl the specified hTER construct). Each hTER truncation (5 ng) was analyzed by Northern analysis after incubation in the RRL (top). The RRL were subjected to an anti-flag immunoprecipitation and assayed for RNA binding by Northern analysis (IP/Northern, middle) and telomerase activity (IP/TRAP, bottom). (A) Assayed for telomerase activity. (B) RNA binding by Northern analysis (as in Figure 2). Lanes 1–12, full-length hTERT synthesized in the presence of the indicated hTER truncation; lanes 13–24, 301-1132 hTERT synthesized in the presence of the indicated hTER truncation, lane 25, full-length hTER with no hTERT protein.