Figure 4.

(A) Effect of RNAi on IFN-induced genes. Real-time RT-PCR was used to compare the content of PKR and MxA transcripts in S1179I cells transfected with siRNA or incubated with IFNα. The relative fold induction of each transcript normalized to that seen in mock-transfected S1179I cells is shown. (B) Determination of expression of OAS and GAPDH mRNA by RT-PCR. (C) Effect of IFN treatment and RNAi on HCV replication was assessed on day 2 after IFN treatment or siRNA transfection in S1179I cells, and compared with mock-transfected S1179I cells. Representative data (mean ± SEM) from two independent experiments are shown.