Figure 2.

Stau1 particles but not Stau1 granules are sensitive to RNase treatment. (A) RNase pretreatment of S16 supernatants released Stau1 from the pellet into the S100. Homogenates (H) were prepared and the resulting S16 was treated with RNase A or mock-treated. Samples were processed as described in Fig. 1. (B) RNase A pretreatment partially disassembled Stau1 particles. RNase A pretreated or mock-treated S100 was analyzed by GF. RNase pretreatment selectively disassembled Stau1 particles (marked by bars) but did not affect the large Staufen granules that contain ribosomes and ER.