. 2003 Feb 5;100(4):2112–2116. doi: 10.1073/pnas.0336359100

Table 1.

Parameters of susceptibility to ex vivo peroxidation of LDL and systemic oxidative stress derived from experimental groups of the study protocol

Mice	LDL lag time, min	LDLTBARS, nmol/mg protein	Plasma isoprostane 8-epi-PGF_2α, pg/ml
ND WT	145 ± 23 (n = 8)	14.3 ± 2.9 (n = 8)	101 ± 16 (n = 6)
HFD WT	113 ± 14 (n = 8)	24.4 ± 4.2 (n = 8)	122 ± 19 (n = 6)
ND p66^Shc−/−	152 ± 23 (n = 8)	13.1 ± 2.8 (n = 8)	93 ± 12 (n = 6)
HFD p66^Shc−/−	138 ± 13 (n = 8)^*	18.2 ± 2.9 (n = 8)^*	105 ± 15 (n = 6)^*

Formation of TBARS at 12 h after exposure of LDL to 1 μM copper sulfate. Lag time represents an index of LDL oxidizability; increased values of lag time reflect increased resistance of LDL to oxidative modification (17, 19).

P < 0.05 vs. 21% HFD-treated WT mice by ANOVA followed by t test and Bonferroni's correction.