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. 2000 Oct;11(10):3353–3364. doi: 10.1091/mbc.11.10.3353

Figure 6.

Figure 6

Rescue of the attachment defect in TSP2-null fibroblasts by treatment with MMP inhibitors. MMP inhibitors BB94 and TIMP-2 were present in cell culture media for 48 h before the attachment assay and in the serum-free media during the attachment to fibronectin-coated tissue culture surfaces. (A) Attachment defect of TSP2-null fibroblasts was corrected by treatment with BB94 in a dose-dependent manner (P < 0.01 for concentrations between 0 and 20 μM). (B) Treatment of fibroblasts with TIMP-2 at concentration of 1 μg/ml restored the attachment of TSP2-null cells to levels nearly equal to those of normal cells. (C) Treatment with a neutralizing antibody to MMP2 also restored attachment of TSP2-null cells to levels approaching those of wild-type cells. A duplicate experiment showed identical results.