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Rescue of the attachment defect in TSP2-null fibroblasts by treatment with exogenous TSP2. Recombinant TSP2 was added to cell culture media, and cells were incubated for 48 h before the attachment assay. Treatment of fibroblasts with TSP2 rescued the attachment defect of TSP2-null cells in a dose-dependent manner (P < 0.01 for all concentrations) and restored the attachment of TSP2-null cells to a normal level at a concentration of 40 nM.
