Figure 8.
Colocalization of ir-CB1 receptors and TH cells in the periventricular hypothalamic nucleus, Bregma −1.8 mm, according to the Paxinos–Watson atlas. (Top) ir-TH cells (red) around the third ventricle. (Middle) ir-CB1 receptors (green) in the same area. (Bottom) Overlay of Top and Middle (400 μm). Arrows indicate two examples of colocalization of TH cells with CB1 receptors. There were a total of seven neurons in the section that had CB1 receptors colocalized to TH cells. Eight TH cells did not have CB1 receptors localized to them; asterisks indicate examples. There were 10 neurons that had CB1 receptors, and only three were not colocalized with TH neurons (arrowhead). (Inset) Magnification of two cells containing ir-TH and ir-CB1 receptors.