Table 1.

Identification of a LEC1 B domain residue required for LEC1 activity

Amino acid substitution in LEC1*	Percent viable seedlings†
M34L	0.8 (110 of 14,400)
R44K	0.6 (82 of 12,800)
H50N	0.7 (176 of 24,000)
D55K	0.01 (5 of 55,600)
N77S	0.4 (48 of 12,000)
Q84K	0.4 (75 of 20,800)
TAE89-91NGD	0.4 (73 of 16,800)
K99T	0.4 (95 of 22,080)

^*Mutant forms of LEC1 are designated with the wild-type amino acid and its position within LEC1, followed by the amino acid that was inserted. cDNA clones encoding LEC1 with the indicated single or triple amino acid substitutions were fused with LEC1 5′ and 3′ flanking sequences and transferred into lec1-1 mutants. 

^† T1 lec1-1 seeds were collected and dried for 2 weeks at 28°C before germination tests were performed. Values obtained reflect the ability of the construct to suppress the lec1 mutation and the transformation efficiency. For the wild-type LEC1 gene, this value was 0.6 (see Fig. 2). The total number of viable seedlings obtained and seeds tested in all experiments are given in parentheses. Independent replicates of transformation experiments gave values similar to the percentages reported here.