Table 2.
A single amino acid substitution confers LEC1 activity to a non-LEC1-type B domain
Construct* | Percent viable seedlings† |
---|---|
LA-NB-LC | 0.03 (8 of 23,600)‡ |
LA-NB-LC (K55D) | 0.23 (70 of 30,400) |
LA-NB-LC consists of A and C domains from LEC1 and the B domain from the non-LEC1-type AHAP3, At4g14540 (see Fig. 2).
Percent viable seedlings was determined as described. Consistent values were obtained from independently repeated experiments. The numbers of seedlings and seeds represent the sum of replicate experiments.
Data from Fig. 2.