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Rho activation occurs in fibroblasts in collagen matrices after LPA stimulation. Fibroblasts were incubated in collagen matrices for 60 min (ATT) followed by 5 min in basal medium (DMEM/5% BSA) (BSA) containing LPA (10 μM) or PDGF (50 ng/ml) as indicated. Samples for each lane represented four matrices containing 4 × 105 cells each. At the end of the incubations, samples were lysed, and aliquots were used to determine GTP-loaded and total levels of Rac and Rho as shown.
