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Metabolic coupling of cells through the fibroblast dendritic network. Donor fibroblasts (labeled with DiI and Calcein AM) and unlabeled cells were incubated 2 h in collagen matrices. After 1 h, 50 ng/ml PDGF (C and D) or 10 μM LPA (E and F) was added. At the end of the incubations, samples were visualized without fixation for DiI (donor cells; A, C, and E) or Calcein AM (donor and recipient cells; B, D, and F). Asterisks indicate recipient cells. Bar, 80 μm.
