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Substrate-specific suppression of doa4 degradation defects by mutations in the DID genes. Pulse-chase analysis of α2 (A), Leu-βgal (B), and Ub-Proβgal (C) in congenic wild-type, doa4Δ, and doa4Δ didΔ strains. For analysis of α2 degradation, cells expressed the transcriptional repressor from the chromosomal MAT locus. For analysis of Leu-βgal and Ub-Proβgal degradation, expression of plasmid-derived fusion proteins was induced with galactose (Bachmair et al., 1986).
