Figure 8.

Model of the budding yeast kinetochore. (A) Revision of the kinetochore model presented in Measday et al. (2002), which now includes Chl4p and Iml3p as part of the outer kinetochore. Mif2p has also been added to the diagram because of its two-hybrid interaction with Chl4p. (B) Proposed molecular architecture of the proteins that have been examined in this article, deduced from requirements for CEN DNA interaction, localization, and protein–protein interactions. Arrows represent requirements for interaction with CEN DNA, beginning with the complex/protein interacting with CEN DNA, and pointed toward the complex/protein required for the interaction. (a) From Ortiz et al. (1999). (b) From Measday et al. (2002).