Table 2.

Genetic interactions between chl4Δ and kinetochore mutants

Straina	Genetic Interactionb
chl4Δ ndc10-42	SLc
chl4Δ ndc10-2	Viable
chl4Δ cep3-1	CSLd
chl4Δ cep3-2	CSLd
chl4Δ ctf13-30	CSLd
chl4Δ mif2-3	SLc
chl4Δ ctf19Δ	Viable
chl4Δ ctf3Δ	Viable
chl4Δ ctf19Δ ctf3Δ	Viable
iml3Δ chl4Δ	Viable
iml3Δ ctf19Δ	Viable
iml3Δ ctf3Δ	Viable
iml3Δ chl4Δ ctf3Δ	Viable
iml3Δ ctf19Δ ctf3Δ	Viable

^aThe strains used in the crosses were chl4Δ (YPH1538 or YPH1539), ndc10-42 (YCTF42), ndc10-2 (YPH1027), cep3-1 (1cAS281), cep3-2 (2bAS282), ctf13-30 (YCTF30), mif2-3 (PMY1002-3A), ctf19Δ (YPH1316), ctf3Δ (YVM111), and iml3Δ (YPH1602). 

^bStrains were incubated at 25°C. 

^cSL, synthetic lethality. Individual mutations do not cause inviability on their own, but a strain containing both mutations is inviable. 

^dCSL, conditional synthetic lethality. Spores are viable at 25°C but die at a lower temperature than the restrictive temperature of the single temperature-sensitive mutant. chl4Δ ctf13-30 double mutants died at 32°C, whereas the nonpermissive temperature of the ctf13-30 single mutant is 35°C; chl4Δ cep3-1 and chl4Δ cep3-2 double mutants were inviable at 31°C and 30°C, respectively, whereas the nonpermissive temperature of the cep3-1 and cep3-2 single mutants is 34°C.