Figure 4.

Fluorescence recover after photobleaching of CFP-clathrin and GFP-AP1 at the trans-Golgi network at 37 and 20°C. HeLa cells at 37°C (A–F) and 20°C (G–L) were imaged for CFP-clathrin (A–C and G–I) and GFP-AP1 (D–F and J–L) before photobleaching (A, D, G, and J), immediately after photobleaching (B, E, H, and K), and either 2 min (C and F) or 5 min (I and L) after photobleaching. The photobleached area is indicated in each figure. Bars, A–F, 11 μm; G–L, 13 μm.