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Fluorescence recovery after photobleaching GFP-AP2 in HeLa cells in cells depleted of K+ (B–D) or treated with hypertonic sucrose (F–H) at 37°C. (A and E) Image of CFP-clathrin and GFP-AP2 in treated HeLa cells. (B and F) AP2 image obtained directly before being photobleached. (C and G) Image immediately after photobleaching. (D and H) Image 2 min after photobleaching. The photobleached area is indicated in each figure. Bars, 5 μm.
