Figure 9.

Fluorescence recovery after photobleaching of CFP-clathrin and GFP-AP1 at the trans-Golgi network of cells depleted of K⁺ or treated with hypertonic sucrose at 37°C. HeLa cells were imaged for CFP-clathrin (A–C and G–I) and GFP-AP1 (D–F and J–L) before photobleaching (A, D, G, and J), immediately after photobleaching (B, E, H, K), and 2 min (C, F, I, L) after photobleaching in cells K⁺ depleted (A–F) or treated with hypertonic sucrose (G–L). The photobleached area is indicated in each figure. Bar, A–F, 7.5 μm; G–L, 5.5 μm.