Table 1.
GFP construct | Condition | Temp, °C | % Recovery | t½ (s) |
---|---|---|---|---|
GFP-AP2 | Control | 37 | 81 ± 8 | 14 ± 4 (n = 15) |
K44A dynamin | 37 | 85 ± 10 | 15 ± 5 (n = 10) | |
Cholesterol depleted | 37 | 86 ± 6 | 16 ± 5 (n = 7) | |
Sucrose | 37 | 93 ± 6 | 19 ± 7 (n = 7) | |
K+ depleted | 37 | 90 ± 9 | 15 ± 5 (n = 11) | |
Control | 15 | 76 ± 11 | 39 ± 10 (n = 9) | |
GFP-clathrin (TGN) | Control | 37 | 83 ± 10 | 14 ± 4 (n = 10) |
Control | 20 | 56 ± 8 | 43 ± 9 (n = 8) | |
GFP-AP1 | Control | 37 | 90 ± 12 | 10 ± 3 (n = 8) |
Control | 20 | 75 ± 10 | 20 ± 6 (n = 8) | |
Sucrose | 37 | 60 ± 3 | 14 ± 4 (n = 6) | |
K+ depleted | 37 | 70 ± 15 | 14 ± 3 (n = 10) | |
GFP-GGA1 | Control | 37 | 77 ± 11 | 12 ± 4 (n = 10) |
Sucrose | 37 | 69 ± 11 | 27 ± 7 (n = 9) | |
K+ depleted | 37 | 77 ± 14 | 16 ± 5 (n = 7) |
The values represent average ± SEM.