Table 1.
Effect of the p38 inhibitor SB203580 on Smad7-induced apoptosis
| Cells | Treatmenta | TUNEL-positive cells/fieldb | DAPI (%)b |
|---|---|---|---|
| PC-3U | None | 0.1 | 0.3 |
| CdCl2 | 2.0 | 0.9 | |
| CdCl2 + SB | 0.2 | 0.6 | |
| SB | 0.4 | 0.5 | |
| Clone I | None | 1.8 | 3.7 |
| CdCl2 | 7.5 | 25.0 | |
| CdCl2 + SB | 1.0 | 0.8 | |
| SB | 0.8 | 0.8 |
a
CdCl2, 1.0 μM CdCl2, 24 h; SB, 10 μM SB203580, 24 h.
b
The number of TUNEL-positive cells were counted in 10 different and randomly chosen fields and the mean number is presented. Nuclei were stained with DAPI 1000 cells were counted in each group. Fragmented and isolated condensed nuclei were counted as apoptotic cells. Investigations were performed using a Zeiss microscope (40× magnification).